C（3〜6か月） Parvalbumin-tTA BAC transgenic mice. tTA expression is detected in PV positive cells (cerebellar Purkinje cells and reticular thalamic nuclei in brain). Kenji TANAKA <a href='https://brc.riken.jp/mus/pcr05770'>Genotyping protocol -PCR-</a> Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> FLP/frt system true B6.Cg-Tg(Pvalb-tTA)1Kftnk B6.Cg-Tg(Pvalb-tTA)1Kftnk RBRC05770 Carrier x Noncarrier [C57BL/6JJcl] Carrier x Noncarrier [C57BL/6JJcl] Neurobiology Research mouse Pvalb genomic DNA (BAC clone RP24-450L20), E. coli tet repressor (tetR), herpes simplex virus (HSV)VP16 activation domain, SV40 polyA additional signal, P1 Phage loxP site, yeast FRT (flipase recombination target) site Tet system Parvalbumin (PV) -tTAトランスジェニックマウス。PV陽性の細胞でtTAを発現するBACトランスジェニックマウス。小脳プルキンエ細胞、視床網様核細胞でtTAが発現する。 PV-tTA, B6-Pvalb-tTA Tg PV-tTA, B6-Pvalb-tTA Tg C (3-6 months) 生理学研究所 田中謙二先生。C57BL/6J受精卵へインジェクションにより作出。C57BL/6と129、CBAの混合背景。 田中　謙二 条件を付加する。<br>利用者は、研究成果の公表にあたって委託者の指定する文献を引用する。Proc Natl Acad Sci U S A., 109(50), 20720-20725 (2012).<br>。学術機関の学術研究に限る。 利用者は提供承諾書を用いて、事前に寄託者へプロジェクト内容を通知する。学術論文へ発表する際は、最初の論文に限り寄託者を共著とする。 Developed by Dr. Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6, 129 and CBA mixed background. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A., 109(50), 20720-20725 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the <br>use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper. Cre/loxP system